Optimum PH

Description

Absorbance at Various pHTime 

Time (sec)

pH 4

pH 5

pH 6

pH 7

pH 8

pH 9

30

0.003

0.025

0.055

0.146

0.005

0.004

60

0.009

0.109

0.152

0.300

0.015

0.008

90

0.0120

0.195

0.255

0.432

0.038

0.010

120

0.020

0.215

0.341

0.552

0.065

0.012

150

0.023

0.333

0.413

0.659

0.081

0.013

180

0.025

0.360

0.478

0.755

0.090

0.013

You just performed an experiment to study the effects of pH buffers on enzyme X. A spectrophotometer set at 450 nm was used to measure absorbance which is when the substrate is converted to its product. Therefore, higher absorbance values suggest higher enzymatic activity. If the experiment was conducted at the same temperature, what is the optimum pH for enzyme X and why? 

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